Biotinylated parathyroid hormone as a probe for the parathyroid hormone receptor
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چکیده
منابع مشابه
Expression of parathyroid hormone-related protein and the parathyroid hormone/parathyroid hormone-related protein receptor in rat thymic epithelial cells.
Thymic epithelial cells are an important source of cytokines and other regulatory peptides which guide thymocyte proliferation and maturation. Parathyroid hormone-related protein (PTHrP), a cytokine-like peptide, has been reported to affect the proliferation of lymphocytes in vitro. The studies presented here were undertaken to test the hypotheses that PTHrP is produced locally within the thymu...
متن کاملParathyroid hormone without parathyroid glands.
In mammals, PTH is a component in a complex signaling system involving three structurally related ligands and two G protein-coupled receptors, which share significant amino acid sequence homology (Fig. 1). PTH is a peptide hormone comprising 84 amino acids that is made by the parathyroid glands (and to a much smaller extend by the thymus) and acts through the PTH/PTH-related peptide (PTHrP) rec...
متن کاملPurification and characterization of a receptor for human parathyroid hormone and parathyroid hormone-related peptide.
The human parathyroid hormone (PTH) receptor (hPTH1R), containing a 9-amino acid sequence of rhodopsin at its C terminus, was transiently expressed in COS-7 cells and solubilized with 0.25% n-dodecyl maltoside. Approximately 18 microg of hPTH1R were purified to homogeneity per mg of crude membranes by single-step affinity chromatography using 1D4, a monoclonal antibody to a rhodopsin epitope. T...
متن کاملTurn-on switch in parathyroid hormone receptor by a two-step parathyroid hormone binding mechanism.
Parathyroid hormone (PTH) and its related receptor (PTHR) are essential regulators of calcium homeostasis and bone physiology. PTH activates PTHR by interacting with a ligand-binding site localized within the N-terminal extracellular domain (the N-domain) and the domain comprising the seven transmembrane helices and the connecting extracellular loops (the J-domain). PTH binding triggers a confo...
متن کاملPTHLH (parathyroid hormone-like hormone)
DNA/RNA Description PTHLP is encoded by a single gene that is highly conserved among species. The gene is composed of 7 exons spanning a region of 13,899 bases (Start: 28,002,284 bp from pter; End: 28,016,183 bp from pter). Orientation: minus strand. The genomic DNA for the PTHLP gene was isolated from a human placental genomic library. Transcription The sequence is supported by 3 sequences fro...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1989
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)84713-0